Fatty Acid Homeostasis Tunes Flagellar Motility by Activating Phase 2 Flagellin Expression, Contributing to Salmonella Gut Colonization.

Long-chain-fatty-acid (LCFA) metabolism is a fundamental cellular process in bacteria that is involved in lipid homeostasis, energy production, and infection. However, the role of LCFA metabolism in Salmonella enterica serovar Typhimurium (. Tm) gut infection remains unclear. Here, using a murine gastroenteritis infection model, we demonstrate involvement of LCFA metabolism in . Tm gut colonization. The LCFA metabolism-associated transcriptional regulator FadR contributes to . Tm gut colonization. deletion alters the gene expression profile and leads to aberrant flagellar motility of . Tm. Colonization defects in the mutant are attributable to altered swimming behavior characterized by less frequently smooth swimming, resulting from reduced expression of the phase 2 flagellin FljB. Notably, changes in lipid LCFA composition by deletion lead to reduced expression of , which is restored by exogenous LCFA. Therefore, LCFA homeostasis may maintain proper flagellar motility by activating expression, contributing to . Tm gut colonization. Our findings improve the understanding of the effect of luminal LCFA on the virulence of enteric pathogens.