Structural Characterization of Polysaccharide and Its Property in Delaying Cellular Senescence.

The sulfated polysaccharide was isolated from the purified polysaccharide (PGP), and its property in delaying HO-induced 2BS cellular senescence was studied. The results showed that PGP was a linear polysaccharide containing alternating α-(1 → 3)- and β-(1 → 4)-galactopyranose units. Most of the sulfate groups are at C6 of the -(1 → 4)-α-D-Galp, and a small part of them are at C3 and C6. PGP pretreatment could decrease SA-β-gal-positive cells and prevent the formation of senescence-associated heterochromatic foci (SAHF) induced by HO in a dose-dependent manner. It is speculated that PGP may delay aging by downregulating the expression of p21 and p53 genes. The finding provides new insights into the beneficial role of . polysaccharide (GP) on retarding senescence process.