The animal species of raw meat and processed meat products was determined by H NMR spectroscopy with subsequent multivariate data analysis. Sample preparation was based on comprehensive lipid extraction to capture nonpolar and polar (amphiphilic) fat components of meat. A nontargeted approach was used to analyze the H NMR data, followed by a principal component analysis, linear discrimination analysis, and cross-validation embedded in a Monte Carlo re-sampling approach. A total of 437 raw meat samples (pork, beef, poultry, and lamb) and 81 processed meat samples (pork, beef, and poultry) were collected to build and/or test the classification model. On average, 98% of the analyzed raw meat samples and 97% of the processed meat products were correctly classified with respect to meat species. Furthermore, relevant spectral regions to identify potential chemical markers such as linoleic acids, trans-fatty acids, and cholesterol for the meat species classification were described.
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