AI Article Synopsis
- - This study examines intraductal papillary neoplasms of the bile duct (IPNB), specifically comparing two types: type 1 and type 2, to identify their differences.
- - Using gene expression analysis and immunohistochemistry on tissue samples, researchers found that type 2 IPNB showed higher levels of DNMT1 protein and more frequent DNA methylation in certain tumor suppressor genes compared to type 1.
- - The findings suggest that measuring DNMT1 protein levels could help differentiate between type 1 and type 2 IPNBs, which could have implications for diagnosis and treatment.
Article Abstract
Background/aim: Intraductal papillary neoplasms of the bile duct (IPNB) are histologically and clinically classified as type 1 and 2. This study aimed to identify the differences between these two types.
Materials And Methods: Based on multiple gene expression analysis (MGEA) using type 1, type 2, and pancreatic intraductal papillary mucinous neoplasms (n=4, 6, and 5, respectively), immunohistochemistry of DNMT1 and methylation-specific PCR for p16, APC, BRCA1, hMLH1, TIMP3, and SOX17 were performed on type 1 and 2 IPNBs (n=14, each).
Results: The DNMT1 protein was highly expressed (p<0.001) in 28.6% of type 1 cases and all type 2 cases. The DNA methylation ratio for the six genes in total as well as for SOX17 was lower in type 1 than in type 2 (p<0.05 each).
Conclusion: Type 2 IPNB showed increased DNMT1 protein expression and increased DNA methylation frequency of the examined tumor suppressor genes compared to type 1. DNMT1 IHC may be helpful in discriminating between these two types.
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| http://dx.doi.org/10.21873/anticanres.15771 | DOI Listing |
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