AI Article Synopsis
- Noncompetitive immunoassays for detecting small molecules, like aflatoxin B1 (AFB1), are shown to be more sensitive than competitive assays.
- A phage-peptide targeting AFB1 was developed using phage-display technology, leading to a new direct noncompetitive magnetic-chemiluminescent enzyme-linked immunoassay (Nc-MCLEIA).
- The Nc-MCLEIA demonstrated significantly improved sensitivity with a limit of detection at 0.006 ng/mL, and achieved accurate recoveries of AFB1 in various food samples, confirming its reliability when compared to HPLC analysis.
Article Abstract
Noncompetitive immunoassays for small molecules are generally considered to be more sensitive than competitive ones. In this study, a phage-peptide against immune complex of aflatoxin B1 (AFB1) and nanobody Nb28 was obtained by phage-display technology. The phage-peptide was labeled with peroxidase and used to develop a direct noncompetitive magnetic-chemiluminescent enzyme-linked immunoassay (Nc-MCLEIA) for AFB1. The 50% signal saturation concentration (SC) and limit of detection (LOD) of Nc-MCLEIA for AFB1 were 0.089 and 0.006 ng/mL, respectively. Compared with competitive immunoassays developed by the Nb28, the sensitivity and efficiency of Nc-MCLEIA were greatly improved. The recoveries of AFB1 from spiked corn, rice, flour, peanut, peanut oil and corn oil samples ranged from 83.8% to 119.2% with coefficient of variable under 8.9%. Furthermore, parallel analysis of natural corn, rice and flour samples by Nc-MCLEIA and HPLC (high performance liquid chromatography) proved that the Nc-MCLEIA was reliable.
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| http://dx.doi.org/10.1016/j.foodchem.2022.133317 | DOI Listing |
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Rapid and sensitive noncompetitive immunoassay for detection of aflatoxin B1 based on anti-immune complex peptide.
Food Chem
November 2022
College of Life Science, Shandong Key Laboratory of Agricultural Microbiology, Shandong Agricultural University, Tai'an 271018, China. Electronic address:
Article Synopsis
- Noncompetitive immunoassays for detecting small molecules, like aflatoxin B1 (AFB1), are shown to be more sensitive than competitive assays.
- A phage-peptide targeting AFB1 was developed using phage-display technology, leading to a new direct noncompetitive magnetic-chemiluminescent enzyme-linked immunoassay (Nc-MCLEIA).
- The Nc-MCLEIA demonstrated significantly improved sensitivity with a limit of detection at 0.006 ng/mL, and achieved accurate recoveries of AFB1 in various food samples, confirming its reliability when compared to HPLC analysis.
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