sp. nov. and sp. nov., isolated from the gut of honeybee ().

Three Gram-stain-positive bacterial strains (designated F502-1, F575-4 and F582-1) were isolated from the gut of honeybee (). These strains were characterized using a polyphasic taxonomic approach. Results of 16S rRNA gene sequence analysis indicated that strain F502-1 was phylogenetically related to the type strains of , and , having 98.7-99.9 % 16S rRNA gene sequence similarities, 84.6-92.5 %  sequence similarities and 95.2-99.0 %  sequence similarities, and that strains F575-4 and F582-1 were closely related to the type strain of , having 99.7 and 99.4 % 16S rRNA gene sequence similarities, respectively. Strains F575-4 and F582-1 had less than 88.7 % and 96.4 % sequence similarities to strain F502-1 and type strains of all species. The average nucleotide identity and digital DNA-DNA hybridization values between strains F502-1, F575-4, F582-1 and type strains of all species were less than 91.3 and 43.5 %, respectively, confirming that they represent two novel species within the genus . Based upon the data obtained in the present study, two novel species, sp. nov. and sp. nov., are proposed and the type strains are F502-1 (=JCM 34500=CCTCC AB 2021026) and F575-4 (=JCM 34501=CCTCC AB 2021028), respectively.