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Response of cecropin transgenesis to challenge with Edwardsiella ictaluri in channel catfish Ictalurus punctatus. | LitMetric

AI Article Synopsis

  • Researchers transferred cecropin B gene constructs from the moth Hyalophora cecropia into channel catfish using electroporation, resulting in two transgenic families with different gene promoters.
  • These transgenic catfish showed improved resistance to the bacterial infection caused by Edwardsiella ictaluri, with significant survival benefits observed at days 3 and 4 after exposure.
  • The study found that while growth rates between transgenic and non-transgenic fish were similar, the genetic modification successfully enhanced disease resistance, highlighting the potential for genetic solutions in fish health management.

Article Abstract

Constructs bearing the cecropin B gene from the moth Hyalophora cecropia, driven by the cytomegalovirus (CMV) promoter, or the common carp beta-actin (β-actin) promoter were transferred to channel catfish, Ictalurus punctatus via electroporation. One F channel catfish family transgenic for cecropin transgene driven by the CMV promoter, and one F channel catfish family transgenic for cecropin transgene driven by the common carp β-actin promoter were produced. F and F individuals exhibited enhanced disease resistance when challenged in tanks with Edwardsiella ictaluri, the causative agent of enteric septicemia of catfish (ESC). Inheritance of the transgene by the F and F generation was 15% and 60%, respectively. Growth rates of the cecropin transgenic and non-transgenic full siblings (controls) channel catfish were not different (P > 0.05). All transgenic fish showed significant resistance to infection by ESC at day 3 and day 4 post exposure (P = 0.005). No correlation was detected between body weight and time to death for all genetic groups (P = 0.34). Results of our study confirmed that genetic enhancement of E. ictaluri resistance can be achieved by cecropin transgenesis in channel catfish. In addition to survival rate, improving survival time is essential because the extension of survival time gives a better chance to apply treatments to stop the bacterial infection.

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Source
http://dx.doi.org/10.1016/j.fsi.2022.05.050DOI Listing

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