AI Article Synopsis
- RipAY is a protein from the bacterium Ralstonia solanacearum that can break down the plant's glutathione (GSH) when activated by specific host proteins called thioredoxins (Trxs).
- Aave_4606, a protein from another bacterium, Acidovorax citrulli, is similar to RipAY and is also predicted to degrade GSH when stimulated by certain host Trxs.
- Research found that Aave_4606's GGCT activity is specifically activated by certain Trxs from Arabidopsis and watermelon, indicating it may play a role in the pathogen's ability to infect its host plants by targeting their cellular defenses.
Article Abstract
RipAY, an effector protein from the plant bacterial pathogen Ralstonia solanacearum, exhibits γ-glutamyl cyclotransferase (GGCT) activity to degrade the host cellular glutathione (GSH) when stimulated by host eukaryotic-type thioredoxins (Trxs). Aave_4606 from Acidovorax citrulli, the causal agent of bacterial fruit blotch of cucurbit plants, shows significant homology to RipAY. Based on its homology, it was predicted that the GGCT activity of Aave_4606 is also stimulated by host Trxs. The GGCT activity of a recombinant Aave_4606 protein was investigated in the presence of various Trxs, such as yeast (ScTrx1), Arabidopsis thaliana (AtTrx-h1, AtTrx-h2, AtTrx-h3, and AtTrx-h5), or watermelon (Cla022460/ClTrx). Unlike RipAY, the GGCT activity of Aave_4606 is stimulated only by AtTrx-h1, AtTrx-h3, AtTrx-h5 and ClTrx from a watermelon, the primary host of A. citrulli, but not by ScTrx1, AtTrx-h2. Interestingly, GGCT activity of Aave_4606 is more efficiently stimulated by AtTrx-h1 and ClTrx than AtTrx-h5. These results suggested that Aave_4606 recognizes host-specific Trxs, which specifically activates the GGCT activity of Aave_4606 to decrease the host cellular GSH. These findings provide new insights into that effector is one of the host-range determinants for pathogenic bacteria via its host-dependent activation.
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| http://dx.doi.org/10.1016/j.bbrc.2022.05.071 | DOI Listing |
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