All eukaryotes possess three DNA-dependent RNA polymerases, Pols I-III, while land plants possess two additional polymerases, Pol IV and Pol V. Derived through duplication of Pol II subunits, Pol IV produces 24-nt short interfering RNAs that interact with Pol V transcripts to target de novo DNA methylation and silence transcription of transposons. Members of the grass family encode additional duplicated subunits of Pol IV and V, raising questions regarding the function of each paralog. In this study, we identify a null allele of the putative Pol IV second subunit, NRPD2, and demonstrate that NRPD2 is the sole subunit functioning with NRPD1 in small RNA production and CHH methylation in leaves. Homozygous nrpd2 mutants have neither gametophytic defects nor embryo lethality, although adult plants are dwarf and sterile.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/tpj.15848 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Development of novel microsatellite marker sets for 5 common tropical hydrozoan species (Cnidaria: Hydrozoa) from the Southwestern Indian Ocean.
Mol Biol Rep
December 2024
Laboratoire d'Excellence CORAIL, Papetoai, Moorea, French Polynesia.
Background: In the context of global change, coral reefs and their associated biodiversity are under threat. Several conservation strategies using population genetics have been explored to protect them. However, some components of this ecosystem are understudied, such as hydrozoans, an important class of benthic organisms worldwide.
View Article and Find Full Text PDFLoss of Hoxa5 function affects Hox gene expression in different biological contexts.
Sci Rep
December 2024
Centre de Recherche sur le Cancer de L'Université Laval, Centre de Recherche du CHU de Québec-Université Laval (Oncology), 1401, 18e Rue, Québec, QC, G1J 1Z4, Canada.
Hoxa5 plays numerous roles in development, but its downstream molecular effects are mostly unknown. We applied bulk RNA-seq assays to characterize the transcriptional impact of the loss of Hoxa5 gene function in seven different biological contexts, including developing respiratory and musculoskeletal tissues that present phenotypes in Hoxa5 mouse mutants. This global analysis revealed few common transcriptional changes, suggesting that HOXA5 acts mainly via the regulation of context-specific effectors.
View Article and Find Full Text PDFTaDL interacts with TaB3 and TaNF-YB1 to synergistically regulate the starch synthesis and grain quality in bread wheat.
J Integr Plant Biol
December 2024
Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Crop Heterosis and Utilization (MOE) and Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.
Starch biosynthesis is a critical factor in wheat (Triticum aestivum L.) quality and yield. However, the full scope of its regulation is not fully understood.
View Article and Find Full Text PDFMultiplex panels of SNP markers based on single-base primer extension in the west Pacific pen shell Atrina lischkeana (Clessin, 1891).
Mol Biol Rep
December 2024
Production Engineering Division, Momoshima Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, 1760 Momoshima, Onomichi, Hiroshima, 722-0061, Japan.
Background: As part of stock enhancement programs for marine fishery species, the stocking of hatchery-produced seedlings into sea areas has been implemented worldwide. DNA markers are vital for responsible stock enhancement practices that aim to conserve the genetic diversity of recipient wild populations. We report novel single-nucleotide polymorphism (SNP) markers and multiplex SNP panels developed for the west Pacific pen shell Atrina lischkeana (Clessin, 1891), a large bivalve that is expected to be a subject of stock enhancement activity as the natural resource has dwindled, especially in Japan.
View Article and Find Full Text PDFA free lunch: microhaplotype discovery in an existing amplicon panel improves parentage assignment for the highly polymorphic Pacific oyster.
G3 (Bethesda)
December 2024
National Cold Water Marine Aquaculture Center, USDA Agricultural Research Service, 483 CBLS, 120 Flagg Road, Kingston, RI 02881, USA.
Amplicon panels using genotyping by sequencing methods are now common, but have focused on characterizing SNP markers. We investigate how microhaplotype (MH) discovery within a recently developed Pacific oyster (Magallana gigas) amplicon panel could increase the statistical power for relationship assignment. Trios (offspring and two parents) from three populations in a newly established breeding program were genotyped on a 592 locus panel.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!