Bacillus subtilis is a widely used model bacterium to study cellular processes and development. The availability of an arrayed mutant library gave us the opportunity to cytologically analyze every mutant and screen for new genes involved in cell shape determination, cell division, and chromosome segregation. Here we describe a high-throughput method to image arrayed B. subtilis mutant libraries using wide-field fluorescence microscopy. We provide a detailed description of growing the arrayed strain collection, preparing slides containing agarose pedestals, setting up the microscopy procedure, acquiring images, and analyzing the images.
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http://dx.doi.org/10.1007/978-1-0716-2221-6_19 | DOI Listing |
J Bacteriol
January 2025
Department of Microbiology, Howard Taylor Ricketts Laboratory, The University of Chicago, Chicago, Illinois, USA.
Unlabelled: Bacteria transport proteins across the plasma membrane to assemble their envelope, acquire nutrients, and establish appropriate interactions with their environment. The majority of these proteins are synthesized as precursors with a cleavable N-terminal signal sequence for recognition by the Sec machinery. In , a small subset of secreted precursors carries a YSIRK/GXXS motif.
View Article and Find Full Text PDFmSystems
January 2025
Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Gluconeogenesis, the reciprocal pathway of glycolysis, is an energy-consuming process that generates glycolytic intermediates from non-carbohydrate sources. In this study, we demonstrate that robust and efficient gluconeogenesis in bacteria relies on the allosteric inactivation of pyruvate kinase, the enzyme responsible for the irreversible final step of glycolysis. Using the model bacterium as an example, we discovered that pyruvate kinase activity is inhibited during gluconeogenesis via its extra C-terminal domain (ECTD), which is essential for autoinhibition and metabolic regulation.
View Article and Find Full Text PDFJ Sci Food Agric
January 2025
University of Agricultural Sciences, Bengaluru, India.
Background: The edible seeds of Ocimum gratissimum and Ocimum basilicum were found to be a potent source of phytochemicals with noteworthy antioxidant, antidiabetic, and antimicrobial properties. This study aimed to investigate the impact of germination and extraction solvents (ethanol (EtOH), distilled water) on the therapeutic properties exhibited and the ability of seed extracts to act as natural food preservatives.
Results: The EtOH extracts of germinated O.
Food Sci Nutr
January 2025
Traditionally fermented sufu is popular because of its flavor, abundance of nutrients, and long shelf life. However, traditional sufu is difficult to produce via industrial processes because of dominant microorganism attenuation during fermentation. Herein, specific protease-producing strains were isolated from traditional sufu.
View Article and Find Full Text PDFThis study was focused on the development of ZnO nanostructures for the efficient oxidation of erythrosine dye and for studying the antibacterial activity of ZnO. It was observed that the phytochemicals from leaves modified the size, shape, crystalline properties and surface chemical composition of the ZnO nanostructures. ZnO nanostructures synthesized with 15 mL leaves extract (S-15) demonstrated highly efficient oxidation of erythrosine dye under the illumination of natural sunlight.
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