Visualizing the Replisome, Chromosome Breaks, and Replication Restart in Bacillus subtilis.

Methods Mol Biol

Centre for Bacterial Cell Biology, Biosciences Institute, Newcastle University, Newcastle upon Tyne, UK.

Published: June 2022

Research over the last two decades has revealed that bacterial genomes are highly organized and that bacteria have sophisticated mechanisms in place to ensure their correct replication and segregation into progeny cells. Here we discuss techniques that can be used with live bacterial cells to analyze DNA replisome dynamics, double-strand chromosome breaks, and restart of repaired replication forks.

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Source
http://dx.doi.org/10.1007/978-1-0716-2221-6_18DOI Listing

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