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Ex vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents. | LitMetric

AI Article Synopsis

  • CGRP (calcitonin gene-related peptide) was discovered in the 1980s and is linked to migraines due to its properties as a vasodilator and neurotransmitter in the trigeminovascular system.
  • A focus on CGRP's role has led to the development of new migraine treatments involving monoclonal antibodies and antagonists targeting CGRP.
  • The text details a protocol for an ex vivo model to study CGRP release from the trigeminovascular system in rodents, outlining methods for measuring the effects of various pharmacological agents and isolating key anatomical structures involved in migraine research.

Article Abstract

Calcitonin gene-related peptide (CGRP) was first discovered in the 1980s as a splice variant from the calcitonin gene. Since its discovery, its role in migraine pathophysiology has been well established, first by its potent vasodilator properties and subsequently by its presence and function as a neurotransmitter in the sensory trigeminovascular system. The migraine-provoking ability of CGRP gave support to the pharma industry to develop monoclonal antibodies and antagonists inhibiting the effect of CGRP. A new treatment paradigm has proven effective in the prophylactic treatment of migraine. One of the useful tools to further understand migraine mechanisms is the ex vivo model of CGRP release from the trigeminovascular system. It is a relatively simple method that can be used with various pharmacological tools to achieve know-how to further develop new effective migraine treatments. The present protocol describes a CGRP release model and the technique to quantify the effect of pharmacological agents on the amount of CGRP released from the trigeminovascular system in rodents. A procedure describing the experimental approach from euthanasia to the measurement of protein levels is provided. The essential isolation of the trigeminal ganglion and the trigeminal nucleus caudalis from both mice and rats and the preparation of rat dura mater are described in detail. Furthermore, representative results from both species (rats and mice) are presented. The technique is a key tool to investigate the molecular mechanisms involved in migraine pathophysiology by using various pharmacological compounds and genetically modified animals.

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Source
http://dx.doi.org/10.3791/63723DOI Listing

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