In the fly brain, neurodegeneration is detected by the presence of vacuoles using conventional hematoxylin and eosin (H&E) or phalloidin staining, which are lengthy and expensive processes. Here, we present a faster and cost-effective 2-day protocol to visualize vacuoles in a fly model of Alzheimer disease. We describe eosin staining in the whole-brain mount, followed by confocal microscopy and image analysis with an open source Fiji plugin. This protocol can be applied to visualize different modules in the fly brain.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136343 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2022.101377 | DOI Listing |
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