AI Article Synopsis
- A novel protein called surface-related antigen (PfSRA) has potential as a malaria vaccine candidate, but its immunogenicity was previously unclear.
- Researchers expressed three fragments of PfSRA and found that immunized mice produced strong specific antibodies with high levels of effectiveness.
- The study confirmed that PfSRA is immunogenic, particularly the F1a fragment, and supports its consideration as a viable vaccine option against malaria.
Article Abstract
, mainly distributed in tropical and subtropical regions of the world, has received widespread attention owing to its severity. As a novel protein, surface-related antigen (PfSRA) has the structural and functional characteristics to be considered as a malaria vaccine candidate; however, limited information is available on its immunogenicity. Here, we expressed three fragments of recombinant PfSRA in an system and further analyzed its immunogenicity. The results showed that rPfSRA-immunized mice produced specific antibodies with high endpoint titers (1:10,000 to 1:5,120,000) and affinity antibodies (i.e., rPfSRA-F1a (97.70%), rPfSRA-F2a (69.62%), and rPfSRA-F3a (91.87%)). In addition, the sera of immunized mice recognized both the native PfSRA and recombinant PfSRA, the rPfSRA antibodies inhibited the invasion of into the erythrocytes, and they were dose-dependent in vitro. This study confirmed PfSRA could be immunogenic, especially the F1a at the conserved region N-terminal and provided further support for it as a vaccine candidate against .
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145071 | PMC |
| http://dx.doi.org/10.3390/pathogens11050550 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
surface-related antigen (PySRA) modulates the host pro-inflammatory responses via binding to CD68 on macrophage membrane.
Infect Immun
May 2024
Department of Public Health and Preventive Medicine, Laboratory of Pathogen Infection and Immunity, Wuxi School of Medicine, Jiangnan University, Wuxi, China.
Malaria, one of the major infectious diseases in the world, is caused by the parasite. antigens could modulate the inflammatory response by binding to macrophage membrane receptors. As an export protein on the infected erythrocyte membrane, surface-related antigen (SRA) participates in the erythrocyte invasion and regulates the immune response of the host.
View Article and Find Full Text PDFImmunogenicity Analysis of the Recombinant Surface-Related Antigen in Mice.
Pathogens
May 2022
Department of Clinical Laboratory, Wuxi 9th Affiliated Hospital of Soochow University (Wuxi 9th People's Hospital), Wuxi 214000, China.
Article Synopsis
- A novel protein called surface-related antigen (PfSRA) has potential as a malaria vaccine candidate, but its immunogenicity was previously unclear.
- Researchers expressed three fragments of PfSRA and found that immunized mice produced strong specific antibodies with high levels of effectiveness.
- The study confirmed that PfSRA is immunogenic, particularly the F1a fragment, and supports its consideration as a viable vaccine option against malaria.
A novel class of Candida glabrata cell wall proteins with β-helix fold mediates adhesion in clinical isolates.
PLoS Pathog
December 2021
Department of Biochemistry, Philipps-Universität, Marburg, Germany.
Candida glabrata is an opportunistic pathogenic yeast frequently causing infections in humans. Though it lacks typical virulence factors such as hyphal development, C. glabrata contains a remarkably large and diverse set of putative wall adhesins that is crucial for its success as pathogen.
View Article and Find Full Text PDFIdentification of the Recombinant Surface-Related Antigen as a Possible Immune Evasion Factor Against Human Splenic Fibroblasts by Targeting ITGB1.
Front Cell Dev Biol
December 2021
Laboratory of Pathogen Infection and Immunity, Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, China.
-infected erythrocytes can enter the spleen and evade spleen clearance to establish chronic infections. However, the mechanism underlying immune evasion in the spleen is still unclear. Human splenic fibroblasts (HSF), also known as barrier cells, play an essential role in the immune function of spleen.
View Article and Find Full Text PDFGenetic Diversity Analysis of Surface-Related Antigen (SRA) in Imported From Africa to China.
Front Genet
August 2021
Laboratory of Pathogen Infection and Immunity, Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, China.
surface-related antigen (SRA) is located on the surfaces of gametocyte and merozoite and has the structural and functional characteristics of potential targets for multistage vaccine development. However, little information is available regarding the genetic polymorphism of . To determine the extent of genetic variation about by characterizing the sequence, 74 samples were collected from migrant workers who returned to China from 12 countries of Africa between 2015 and 2019.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!