AI Article Synopsis
- sp. alg1 produces two alginate lyases, AlyFRA and AlyFRB, that convert alginate into the monosaccharide 4-deoxy-L-5-hexoseulose uronic acid (DEH), which is further metabolized to pyruvate.
- The study involved preparing recombinant versions of these enzymes and assessing their ability to produce DEH, achieving a 96.8% conversion rate from sodium alginate.
- The research demonstrated that immobilized AlyFRA and AlyFRB maintained a 79.6% DEH yield even after being reused seven times, highlighting a cost-effective and efficient method for producing DEH from brown algae for biofuel applications.
Article Abstract
sp. alg1 expresses two alginate lyases, AlyFRA and AlyFRB, to produce the linear monosaccharide 4-deoxy-L--5-hexoseulose uronic acid (DEH) from alginate, metabolizing it to pyruvate. In this study, we prepared recombinant AlyFRA and AlyFRB and their immobilized enzymes and investigated DEH production. Purified AlyFRA and AlyFRB reacted with sodium alginate and yielded approximately 96.8% DEH. Immobilized AlyFRA and AlyFRB were prepared using each crude enzyme solution and -carrageenan, and immobilized enzyme reuse in batch reactions and DEH yield were examined. Thus, DEH was produced in a relatively high yield of 79.6%, even after the immobilized enzyme was reused seven times. This method can produce DEH efficiently and at a low cost and can be used to mass produce the next generation of biofuels using brown algae.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144776 | PMC |
| http://dx.doi.org/10.3390/molecules27103308 | DOI Listing |
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Production of 4-Deoxy-L--5-Hexoseulose Uronic Acid Using Two Free and Immobilized Alginate Lyases from sp. Alg1.
Molecules
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Graduate School of Bioresources, Mie University, 1577 Kurimamachiya-cho, Tsu 514-8507, Mie, Japan.
Article Synopsis
- sp. alg1 produces two alginate lyases, AlyFRA and AlyFRB, that convert alginate into the monosaccharide 4-deoxy-L-5-hexoseulose uronic acid (DEH), which is further metabolized to pyruvate.
- The study involved preparing recombinant versions of these enzymes and assessing their ability to produce DEH, achieving a 96.8% conversion rate from sodium alginate.
- The research demonstrated that immobilized AlyFRA and AlyFRB maintained a 79.6% DEH yield even after being reused seven times, highlighting a cost-effective and efficient method for producing DEH from brown algae for biofuel applications.
Falsirhodobacter sp. alg1 Harbors Single Homologs of Endo and Exo-Type Alginate Lyases Efficient for Alginate Depolymerization.
PLoS One
July 2017
Faculty of Science and Engineering, Waseda University, Tokyo, Japan.
Alginate-degrading bacteria play an important role in alginate degradation by harboring highly efficient and unique alginolytic genes. Although the general mechanism for alginate degradation by these bacteria is fairly understood, much is still required to fully exploit them. Here, we report the isolation of a novel strain, Falsirhodobacter sp.
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