AI Article Synopsis
- The study discusses the creation of a recombinant baculovirus vector system for producing foreign proteins and gene delivery.
- The researchers constructed the BmBac-UPS and BmBac-Gal4 vectors by combining specific promoter and coding regions in a pFastBac Dual vector.
- The results confirmed that these recombinant viruses effectively formed polyhedra and demonstrated increased pathogenicity when infecting silkworm larvae, highlighting their potential use in gene delivery applications.
Article Abstract
The baculovirus vector expression system is a well-established tool for foreign protein production and gene delivery. In this study, we constructed a recombinant baculovirus vector system. The UAS promotor region and nucleopolyhedrovirus (BmNPV) polyhedrin coding region were ligated into a pFastBac Dual vector to obtain a BmBac-UPS recombinant bacmid. The recombinant bacmid BmBac-Gal4 was generated by the same strategy which has a Gal4 coding region controlled by the IE2 promoter. BmBac-UPS and BmBac-IGal4 were co-infected into silkworm BmN cells to confirm the ability of the UAS/Gal4 system to form polyhedrons in cells. Furthermore, the recombinant viruses were tested for infection efficiency and the ability to generate polyhedra in transgenic cell line BmE. The results showed that recombinant viruses have the ability to form polyhedrons and gain raised pathogenicity when orally infected larvae and are applied as the preferred tool for foreign gene delivery and expression.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143162 | PMC |
| http://dx.doi.org/10.3390/microorganisms10051013 | DOI Listing |
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