AI Article Synopsis
- Metabolic disorders are linked to disrupted circadian rhythms, and certain bioactive compounds can influence lipid metabolism through these rhythms.
- Piperine (PIP), found in black pepper, can help reduce obesity by affecting lipid metabolism but the involvement of circadian clock genes in this effect is still unclear.
- In experiments with HepG2 cells, PIP was shown to improve mitochondrial function and circadian synchronization while reducing oxidative stress, potentially through specific signaling pathways related to lipid regulation.
Article Abstract
Metabolic disorders are closely associated with the dysregulation of circadian rhythms. Many bioactive components with lipid metabolism-regulating effects have been reported to function through circadian clock-related mechanisms. As the main pungent principle of black pepper, piperine (PIP) has been demonstrated to possess anti-obesity bioactivity by affecting hepatic lipid metabolism-related factors. However, whether the circadian clock genes and are involved in the protective effect of PIP against lipid metabolism disorders remains unknown. In this work, oleic acid (OA) induced lipid accumulation in HepG2 cells. The effect of PIP on redox status, mitochondrial functions, and circadian rhythms of core clock genes were evaluated. Results revealed that PIP alleviated circadian desynchrony, ROS overproduction, and mitochondrial dysfunction. A mechanism study showed that PIP could activate the SREBP-1c/PPARγ and AMPK/AKT-mTOR signaling pathways in a /-dependent manner in HepG2 cells. These results indicated that and played important roles in the regulating effect of PIP on hepatic lipid homeostasis.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144199 | PMC |
| http://dx.doi.org/10.3390/ijms23105611 | DOI Listing |
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