Due to the prevailing ambiguity regarding the performance of rapid antigen tests (RATs) for B.1.1.529 (Omicron) variant diagnosis, a commercial RAT was evaluated in the emergency ward of a general hospital in Larissa, Central Greece. The sampling and the evaluation were repeated twice by different personnel. Discordance between the two samplings was observed regarding the sensitivity (47.5%, 95% CI: 39.0-56.1 vs. 78.6%, 95% CI: 69.1-86.2) and specificity (93.8%, 95% CI: 86.0-97.9 vs. 100.0%, 95% CI: 93.3-100.0) of the RAT. Furthermore, the test displayed slightly lower sensitivity (78.6% vs. 85.5%, 95% CI: 79.1-90.5) compared to its initial evaluation that was conducted by our team when the B.1.1.7 (Alpha) variant was dominant.
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http://dx.doi.org/10.3390/diagnostics12051048 | DOI Listing |
Viruses
January 2025
Microbiology Laboratory, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
Aims: The screening and diagnosis of dengue virus infection play a crucial role in controlling the epidemic of dengue fever, highlighting the urgent need for a highly sensitive, simple, and rapid laboratory testing method. This study aims to assess the clinical performance of MAGLUMI Denv NS1 in detecting dengue virus NS1 antigen.
Methods: A retrospective study was conducted to assess the sensitivity and specificity of MAGLUMI Denv NS1 using residual samples.
Pathogens
January 2025
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 'G. Caporale', National Reference Center for Brucellosis, 64100 Teramo, Italy.
Rose Bengal antigen and smooth lipopolysaccharide (s-LPS) were produced from a field strain of ("homologous" antigens) and from the reference strain S99 ("heterologous" antigens); they are currently used for the diagnosis of brucellosis in cattle, water buffaloes, sheep, goats, and pigs, as recommended in the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals of the World Organization for Animal Health (WOAH). "Homologous" and "heterologous" antigens were used in a rapid serum agglutination test (Rose Bengal test, RBT) and a competitive ELISA assay (c-ELISA) to test a panel of sera, blood, and other body fluids (cerebrospinal fluid, pericardial fluid, tracheal fluid, and aqueous humor) collected from 71 individuals belonging to five cetacean species (; ; ; ; and ), which were found stranded on the Italian coastline. Six animals were positive for spp.
View Article and Find Full Text PDFJ Clin Med
January 2025
Department of Health Sciences (DISSAL), University of Genoa, 16126 Genoa, Italy.
In immunocompromised patients, pneumonia presents a diagnostic challenge due to diverse etiologies, nonspecific symptoms, overlapping radiological presentation, frequent co-infections, and the potential for rapid progression to severe disease. Thus, timely and accurate diagnosis of all pathogens is crucial. This narrative review explores the latest advancements in microbiological diagnostic techniques for pneumonia in immunocompromised patients.
View Article and Find Full Text PDFInt J Mol Sci
January 2025
Institute of Molecular Biology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. Bl. 21, 1113 Sofia, Bulgaria.
Replication forks encounter various impediments, which induce fork stalling and threaten genome stability, yet the precise dynamics of fork stalling and restart at the single-cell level remain elusive. Herein, we devise a live-cell microscopy-based approach to follow hydroxyurea-induced fork stalling and subsequent restart at 30 s resolution. We measure two distinct processes during fork stalling.
View Article and Find Full Text PDFMicroorganisms
January 2025
Medical Faculty, Sofia University "St. Kliment Ohridski", 1407 Sofia, Bulgaria.
Recently a resurgence of has arisen, with concerns around the highly virulent M1 lineage. Our aim was to characterize , the immune responses it causes, and to determine the presence of the M1 lineage in Sofia, Bulgaria. In our study, the infections were confirmed by culture testing or rapid antigen test.
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