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Challenges in Quantifying 8-OHdG and 8-Isoprostane in Exhaled Breath Condensate. | LitMetric

AI Article Synopsis

  • Exhaled breath condensate (EBC) is a promising non-invasive method for assessing airway inflammation and has been linked to biomarkers for lung diseases like asthma and COPD.
  • * Biomarkers 8-Hydroxydesoxyguanosine (8-OHdG) and 8-isoprostane were analyzed in EBC using a new sensitive chemical method, but they were undetectable in samples from healthy and asthmatic subjects.
  • * The study highlights concerns about the reliability of EBC collection methods, suggesting that more work is needed to improve the accuracy of these biomarkers before they can be widely used.

Article Abstract

Exhaled breath condensate (EBC) has attracted substantial interest in the last few years, enabling the assessment of airway inflammation with a non-invasive method. Concentrations of 8-Hydroxydesoxyguanosine (8-OHdG) and 8-isoprostane in EBC have been suggested as candidate biomarkers for lung diseases associated with inflammation and oxidative stress. EBC is a diluted biological matrix and consequently, requires highly sensitive chemical analytic methods (picomolar range) for biomarker quantification. We developed a new liquid chromatography coupled to tandem mass spectrometry method to quantify 8-OHdG and 8-isoprostane in EBC simultaneously. We applied this novel biomarker method in EBC obtained from 10 healthy subjects, 7 asthmatic subjects, and 9 subjects with chronic obstructive pulmonary disease. Both biomarkers were below the limit of detection (LOD) despite the good sensitivity of the chemical analytical method (LOD = 0.5 pg/mL for 8-OHdG; 1 pg/mL for 8-isoprostane). This lack of detection might result from factors affecting EBC collections. These findings are in line with methodological concerns already raised regarding the reliability of EBC collection for quantification of 8-OHdG and 8-isoprostane. Precaution is therefore needed when comparing literature results without considering methodological issues relative to EBC collection and analysis. Loss of analyte during EBC collection procedures still needs to be resolved before using these oxidative stress biomarkers in EBC.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138069PMC
http://dx.doi.org/10.3390/antiox11050830DOI Listing

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