AI Article Synopsis
- Researchers developed animal models of traumatic pancreatitis (TP) in rats to study how exosomes from umbilical cord mesenchymal stem cells (ucMSC-Ex) help with treatment.
- The study involved 64 rats divided into eight groups, with varying levels of pancreatic damage and some receiving ucMSC-Ex treatment, and they used different techniques to measure tissue injury, enzyme activity, and apoptosis.
- Results showed that treatment with ucMSC-Ex significantly reduced pancreatic injury, lowered inflammatory markers, and decreased cell apoptosis compared to untreated TP groups, suggesting a protective effect on the pancreas.
Article Abstract
The animal models of traumatic pancreatitis (TP) were established to evaluate the specific mechanisms by which umbilical cord mesenchymal stem cell-derived exosomes (ucMSC-Ex) exert therapeutic effects. Sixty four rats were randomly divided into eight groups, including TP groups with three different degrees and relevant groups with ucMSC-Ex treated. The degrees of pancreatic tissue injury were evaluated by Histological Examination. Furthermore, enzyme-linked immunosorbent assay were applied to evaluate the activity of pancreatic enzymes and levels of inflammatory factors in serum. Finally, the apoptotic effects of each group were evaluated by TUNEL, western blot (WB), and real time fluorescence quantitative polymerase chain reaction (RT-qPCR). The pancreatic histopathological score and serum amylase and lipase levels gradually increased in various degrees of TP and the levels in the treatment group were all significantly decreased. The apoptosis index gradually increased in each TP group and significantly decreased in the treatment group in TUNEL results. WB and RT-qPCR showed the same trend, that bax and caspase-3 gradually increased and bcl-2 gradually decreased in TP groups. Compared with TP groups, the expression of bax and caspase-3 were lower while bcl-2 expression was higher in the treatment group. ucMSC-Ex suppressed the inflammatory response and inhibited pancreatic acinar cell apoptosis to promote repair of injured pancreatic tissue.
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| http://dx.doi.org/10.1007/s10495-022-01732-1 | DOI Listing |
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