Single bacteria detection by droplet DNAzyme-coupled rolling circle amplification.

Anal Methods

School of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), Dalian University of Technology, Dalian POCT Laboratory, Dalian, 116024, China.

Published: June 2022

We described a new system termed droplet DNAzyme-coupled rolling circle amplification (dDRCA) that can selectively detect bacteria from clinical urine samples with single-cell sensitivity within 1.5 h compared with the several hours needed for traditionally used culture-based methods.

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Source
http://dx.doi.org/10.1039/d2ay00656aDOI Listing

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