[Cloning and tissue expression analysis of the gene of ].

Leptin receptor overlapping transcript (LepROT) plays multiple roles in the regulation of immune systems. However, very little information is available about the anti-infectious mechanisms of amphibians LepROT. In this study, the cDNA sequence of the gene was determined by using RT-PCR and bioinformatics analysis. Then, the () and lipopolysaccharides (LPS) infected models of was constructed to obtain histopathological characteristics. Constitutive expression of mRNA and NF-κB signaling pathway were detected by real-time quantitative PCR. The full-length cDNA of gene was 396 bp and encoded 131 amino acids. Amino acid sequence analysis revealed LepROT shares 93.74% and 86.39% identity with homologues from other amphibians and mammals respectively, and the gene was quite conserved among different species. After infection, the relative expression levels of , , and mRNA were all significantly upregulated ( < 0.01), but showed a diverse temporal pattern of up-regulation in different tissues. Therefore, it was proposed that the gene of . might activate the NF-κB signaling pathway to exert anti-infectious effects, thus providing evidence for further extending the biological function of LepROT.