AI Article Synopsis
- Circular RNAs (circRNAs) have been found in colorectal cancer (CRC), with a study identifying 207 up-regulated and 357 down-regulated circRNAs, aiming to understand their roles in cancer biology.
- The research involved techniques like microarray and qRT-PCR to determine the expression of circRNAs, leading to the discovery of six circRNAs with high diagnostic potential for CRC.
- A regulatory network was created involving circRNAs, miRNAs, and mRNAs, offering insights through bioinformatics analyses, which could help in identifying new mechanisms and clinical targets for CRC treatment.
Article Abstract
Background: Circular RNAs (circRNAs) have been discovered in colorectal cancer (CRC), but there are few reports on the expression distribution and functional mining analysis of circRNAs.
Methods: Differentially expressed circRNAs in CRC tissues and adjacent normal tissues were screened and identified by microarray and qRT-PCR. ROC curves of the six circRNAs were analyzed. A series of bioinformatics analyses on differentially expressed circRNAs were performed.
Results: A total of 207 up-regulated and 357 down-regulated circRNAs in CRC were screened, and three top up-regulated and down-regulated circRNAs were chosen to be verified in 33 pairs of CRCs by qRT-PCR. 6 circRNAs showed high diagnostic values (AUC = 0.6860, AUC = 0.8127, AUC = 0.7502, AUC = 0.9945, AUC = 0.9642, AUC = 0.9486 for hsa_circRNA_100833, hsa_circRNA_103828, hsa_circRNA_103831 and hsa_circRNA_103752, hsa_circRNA_071106, hsa_circRNA_102293). A circRNA-miRNA-mRNA regulatory network (cirReNET) including six candidate circRNAs, 19 miRNAs and 210 mRNA was constructed, and the functions of the cirReNET were predicted and displayed via Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses on these mRNAs and protein-protein interaction (PPI) network of the hub genes acquired by string and CytoHubba.
Conclusion: A cirReNET containing potential diagnostic and predictive indicators of CRCs and several critical circRNA-miRNA-mRNA regulatory axes (cirReAXEs) in CRC were mined, and may provide a novel route to study the mechanism and clinical targets of CRC.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9124462 | PMC |
| http://dx.doi.org/10.7717/peerj.13420 | DOI Listing |
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