MNSs-mediated N-glycan processing is essential for auxin homeostasis in roots during alkaline response.

iScience

Center for Plant Water-use and Nutrition Regulation and College of Life Sciences, Joint International Research Laboratory of Water and Nutrient in Crop and College of Resource and Environment, Fujian Agriculture and Forestry University, Jinshan, Fuzhou 350002, China.

Published: May 2022

Early steps in the endoplasmic reticulum (ER) lumen and -Golgi comprise trimming of N-glycans by class I α-mannosidases (MNSs) play crucial roles in root growth and stress response. Herein, we found that the root growth inhibition in the mutant was partially rescued under alkaline condition, and inhibitor treatment to disrupt auxin transport counteracted this alkaline-maintained root growth. Further study showed that indole-3-acetic acid (IAA) levels were undetectable in at normal condition and recovered at alkaline condition, which corroborate our N-glycopeptide profiling, from which N-glycopeptides related with IAA biosynthesis, amino acid conjugates hydrolysis, and response showed differential abundance between normal and alkaline conditions in . Overall, our results linked the need for MNSs-mediated N-glycan processing in the ER and -Golgi with maintenance of auxin homeostasis and transport in roots during the response to alkaline stress.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118167PMC
http://dx.doi.org/10.1016/j.isci.2022.104298DOI Listing

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