AI Article Synopsis
- A protocol is outlined for creating organoids from human thyroid cancer cells using patient-derived cells.
- Both organoids and primary cell lines are established, with the organoid medium enhanced by conditioned medium from the primary line.
- This approach allows organoids to be cultured for up to 10 months while preserving their original genetic and phenotypic traits.
Article Abstract
Here, we describe a protocol to generate organoids from human thyroid cancer cells. Starting from the same patient-derived cells, we establish both organoids and primary lines. The organoid medium is supplemented with conditioned medium obtained from the primary cell line. This modification enables culture of the organoid lines for up to 10 months. Even after long-term culture, the organoids retain the genetic and phenotypic characteristics of their tissue of origin.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9115321 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2022.101393 | DOI Listing |
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