root cells developing into syncytia through the parasitic activities of the pathogenic nematode underwent isolation by laser microdissection (LM). Microarray analyses have identified the expression of a () homolog in syncytia undergoing parasitism but during a defense response. encodes part of the common symbiosis pathway (CSP) involving , and other CSP genes. The identified gene expression, and symbiosis role, suggests the possible existence of commonalities between symbiosis and defense. has 3 , 12 , and 2 paralogs. LM-assisted gene expression experiments of isolated syncytia under further examination here show , and expression occurring during the defense response in the -resistant genotypes . and . indicating a broad and consistent level of expression of the genes. Transgenic overexpression (OE) of , and impairs parasitism. RNA interference (RNAi) of , and increases parasitism. The combined opposite outcomes reveal a defense function for these genes. Prior functional transgenic analyses of the 32-member () gene family has determined that 9 of them act in the defense response to parasitism, referred to as defense s. RNA-seq analyses of root RNA isolated from the 9 defense s undergoing OE or RNAi reveal they alter the relative transcript abundances (RTAs) of specific , and paralogs. In contrast, transgenically-manipulated , and expression influences and RTAs under certain circumstances. The results show homologs of the CSP, and defense pathway are linked, apparently involving co-regulated gene expression.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114929 | PMC |
http://dx.doi.org/10.3389/fpls.2022.842597 | DOI Listing |
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