AI Article Synopsis

  • The study examines how root cells transform into syncytia due to pathogenic nematode activity, isolating these cells using laser microdissection for analysis.
  • Microarray data reveals that certain genes involved in the common symbiosis pathway also play a role in defense against parasitism, suggesting overlapping functions between symbiosis and defense mechanisms.
  • Manipulating the expression of specific genes through transgenic overexpression or RNA interference demonstrates that these genes have a dual role in defending against nematode parasitism, altering gene expression patterns in response to different genetic alterations.

Article Abstract

root cells developing into syncytia through the parasitic activities of the pathogenic nematode underwent isolation by laser microdissection (LM). Microarray analyses have identified the expression of a () homolog in syncytia undergoing parasitism but during a defense response. encodes part of the common symbiosis pathway (CSP) involving , and other CSP genes. The identified gene expression, and symbiosis role, suggests the possible existence of commonalities between symbiosis and defense. has 3 , 12 , and 2 paralogs. LM-assisted gene expression experiments of isolated syncytia under further examination here show , and expression occurring during the defense response in the -resistant genotypes . and . indicating a broad and consistent level of expression of the genes. Transgenic overexpression (OE) of , and impairs parasitism. RNA interference (RNAi) of , and increases parasitism. The combined opposite outcomes reveal a defense function for these genes. Prior functional transgenic analyses of the 32-member () gene family has determined that 9 of them act in the defense response to parasitism, referred to as defense s. RNA-seq analyses of root RNA isolated from the 9 defense s undergoing OE or RNAi reveal they alter the relative transcript abundances (RTAs) of specific , and paralogs. In contrast, transgenically-manipulated , and expression influences and RTAs under certain circumstances. The results show homologs of the CSP, and defense pathway are linked, apparently involving co-regulated gene expression.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114929PMC
http://dx.doi.org/10.3389/fpls.2022.842597DOI Listing

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