The natural substance class of terpenoids covers an extremely wide range of different structures, although their building block repertoire is limited to the C compounds DMAPP and IPP. This study aims at the characterization of methyltransferases (MTases) that modify these terpene precursors and the demonstration of their suitability for biotechnological purposes. All seven enzymes tested accepted IPP as substrate and altogether five C compounds and six C compounds were formed within the reactions. A high selectivity for the deprotonation site as well as high stereoselectivity could be observed for most of the biocatalysts. Only the enzyme from Micromonospora humi also accepted DMAPP as substrate, converting it into (2R)-2-methyl-IPP in vitro. In vivo studies demonstrated the production of a C compound and a hydride shift step within the MTase-catalyzed reaction. Our study presents IPP/DMAPP MTases with very different catalytic properties, which provide biosynthetic access to many novel terpene-derived structures.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9401056 | PMC |
| http://dx.doi.org/10.1002/cbic.202200091 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Stress-induced self-assembly of hierarchically twisted stripe arrays.
Sci Bull (Beijing)
December 2024
Department of Chemistry, Laboratory of Advance Materials, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, State Key Laboratory of Molecular Engineering of Polymers, and iChEM, Fudan University, Shanghai 200433, China. Electronic address:
Hierarchical organization is prevalent in nature, yet the artificial construction of hierarchical materials featuring asymmetric structures remains a big challenge. Herein, we report a stress-induced self-assembly strategy for the synthesis of hierarchically twisted stripe arrays (HTSAs) with mesoporous structures. A soft and thin mesostructured film assembled by micelles and TiO oligomers is the prerequisite.
View Article and Find Full Text PDFEfficient recombinant protein production requires mammalian stable cell lines or often relies on inefficient transfection processes. Baculoviral transduction of mammalian cells (BacMam) offers cost-effective and robust gene transfer and straightforward scalability. The advantages over conventional approaches are, no need of high biosafety level laboratories, efficient transduction of various cell types and transfer of large transgenes into host cells.
View Article and Find Full Text PDFHigh-throughput screening of acetogenic strains for growth and metabolite profiles on readily available biomass.
Bioresour Technol
January 2025
Centre for Industrial Biotechnology and Biocatalysis (InBio.be), Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Ghent, 9000, Belgium; Bio Base Europe Pilot Plant (BBEPP), Rodenhuizekaai 1, Ghent, 9042, Belgium. Electronic address:
Abundant biomass, including industrial waste streams and second-generation (2G) and third-generation (3G) feedstocks, offers significant potential for sustainable bioconversion, nevertheless challenges such as fermentation inhibitors, CO losses and substrate selectivity of traditional microbial hosts hinder process efficiency. In this study, we address these challenges by exploring acetogenic bacteria as alternative microbial hosts. Using a newly established high-throughput method, acetogens were evaluated for their capacity to hydrolyse and metabolise variety of substrates derived from 2G and 3G feedstocks and industrial waste streams.
View Article and Find Full Text PDF3D Printing Organogels with Bioderived Cyrene for High-Resolution Customized Hydrogel Structures.
Langmuir
January 2025
Surface Science and Bio-nanomaterials Laboratory, Department of Chemical Engineering, University of Waterloo, Waterloo, Ontario N2L 3G1 Canada.
3D printing techniques are increasingly being explored to produce hydrogels, versatile materials with a wide range of applications. While photopolymerization-based 3D printing can produce customized hydrogel shapes and intricate structures, its reliance on rigid printing conditions limits material properties compared to those of extrusion printing. To address this limitation, this study employed an alternative approach by printing an organogel precursor using vat polymerization with organic solvents instead of water, followed by solvent exchange after printing to create the final hydrogel material.
View Article and Find Full Text PDFAuto-RapTAC: A Versatile and Sustainable Platform for the Automated Rapid Synthesis and Evaluation of PROTAC.
J Med Chem
January 2025
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
The tedious synthesis and limited throughput biological evaluation remain a great challenge for discovering new proteolysis targeting chimera (PROTAC). To rapidly identify potential PROTAC lead compounds, we report a platform named Auto-RapTAC. Based on the modular characteristic of the PROTAC molecule, a streamlined workflow that integrates lab automation with "click chemistry" joint building-block libraries was constructed.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!