According to the chemiluminescence characteristics of the luminol-hydrogen peroxide (HO) system, this work designed a novel and effective electrochemiluminescence (ECL) aptasensor to detect atrazine (ATZ) rapidly. Silver nanoparticles (AgNPs) could effectively catalyze the decomposition of HO and enhance the ECL intensity of the luminol-HO system. Once ATZ was modified on the aptasensor, the ECL intensity was significantly weakened because of the specific combination between ATZ and its aptamer. Therefore, the changes in ECL intensity could be used to detect the concentration of ATZ. Under optimal detecting conditions, the aptasensor had a wide linear range from 1 × 10 ng/mL to 1 × 10 ng/mL and a low limit of detection (3.3 × 10 ng/mL). The designed aptasensor had the advantages of good stability, reproducibility, and specificity. The aptasensor could be used to detect the ATZ content of tap water, soil, and cabbage and had satisfactory results. This work effectively constructs a novel, effective, and rapid ECL aptasensor for detecting ATZ in actual samples.
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http://dx.doi.org/10.3390/s22093430 | DOI Listing |
Anal Chem
January 2025
National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
, a significant zoonotic pathogen, annually caused substantial economic losses in the swine industry and had intensified threat to public health due to the recent emergence of human-associated clade. In this study, we discovered that the rare-earth metal-based metal-organic frameworks (Y-BTC) possessed excellent ECL capabilities. After prereduction at high voltage, its ECL intensity was enhanced by two times.
View Article and Find Full Text PDFAnal Chem
January 2025
School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, P R China.
Serum amyloid A (SAA) is a key biomarker for diagnosing inflammatory responses in diseases like influenza and COVID-19. An electrochemiluminescence (ECL) biosensor has been constructed for signal enhancement in SAA detection by encapsulating 4,4',4″,4‴-(1,3,6,8-pyrenetetrayl) tetrakis-benzoic acid (TBAPy) into liposomes. Such biomimetic encapsulation shields the biologically important membrane to avoid aggregation of TBAPy and prevents quenching.
View Article and Find Full Text PDFBiosens Bioelectron
December 2024
Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China. Electronic address:
Multi-signal-based self-calibrating biosensors have become a research focus due to their superior accuracy and sensitivity in recent years. Herein, the potential-resolved differential ECL immunoassay based on dual co-reactants regulation was developed. Meso-tetra(4-carboxyphenyl)porphyrin (TCPP) functionalized zirconium dioxide (ZrO) composites (TCPP-ZrO) was first synthesized using TCPP as the luminophore and ZrO as the enhancer and stabilizer.
View Article and Find Full Text PDFMolecules
January 2025
School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China.
Dopamine (DA) is an important catecholamine neurotransmitter and its abnormal concentration is closely related to diseases such as hypertension, Parkinson's disease and schizophrenia. Due to the advantages of high sensitivity and fast response for electrochemiluminescence (ECL), developing ECL sensors for detecting DA was very critical in clinical diagnosis. ECL resonance energy transfer (ECL-RET) was an effective signaling mechanism.
View Article and Find Full Text PDFJ Colloid Interface Sci
January 2025
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China. Electronic address:
Covalent organic frameworks (COFs) with highly ordered structures and predictable optoelectronic properties provide an ideal platform to investigate the electrochemiluminescence (ECL) performance based on organic materials by atomically varying the molecular construction. Herein, the effect of imine-bond orientation on the ECL performance of COFs is investigated. We report two COFs (NC-COF and CN-COF) with different orientations of imine bonds using pyrene donor units (D) and bipyridine acceptor motifs (A) monomers.
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