AI Article Synopsis
- RNP condensates made up of mRNAs and proteins play important roles in various cell types, but their behavior during aging in neurons isn't well studied.
- High-resolution imaging in aging Drosophila brains shows that RNP components group into large, dynamic granules, with increases linked to the helicase Me31B/DDX6 and requiring PKA kinase activity.
- This clustering leads to age-dependent translational repression of certain mRNAs, suggesting that these changes may impact gene expression as organisms age.
Article Abstract
Cytoplasmic RNP condensates enriched in mRNAs and proteins are found in various cell types and associated with both buffering and regulatory functions. While a clear link has been established between accumulation of aberrant RNP aggregates and progression of aging-related neurodegenerative diseases, the impact of physiological aging on neuronal RNP condensates has never been explored. Through high-resolution imaging, we uncover that RNP components progressively cluster into large yet dynamic granules in the aging Drosophila brain. We further show that age-dependent clustering is caused by an increase in the stoichiometry of the conserved helicase Me31B/DDX6, and requires PKA kinase activity. Finally, our functional analysis reveals that mRNA species recruited to RNP condensates upon aging exhibit age-dependent translational repression, indicating that co-clustering of selected mRNAs and translation regulators into repressive condensates may contribute to the specific post-transcriptional changes in gene expression observed in the course of aging.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120078 | PMC |
| http://dx.doi.org/10.1038/s41467-022-30066-4 | DOI Listing |
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