AI Article Synopsis
- Researchers developed a new substrate for cartilage repair by crosslinking polyvinyl alcohol hydrogel chains with polyurethane and conducting tests on a sheep model.
- In vitro tests showed that chondrocyte cells could produce proteoglycan on the substrate, and gene expression analysis revealed important markers for cartilage formation.
- The study confirmed that the new substrate helped create neocartilage tissue in vivo, suggesting its potential usefulness in cartilage tissue engineering.
Article Abstract
In this study, polyvinyl alcohol hydrogel chains were crosslinked by polyurethane in order to synthesize a suitable substrate for cartilage lesions. The substrate was fully characterized, and in vitro and in vivo investigations were conducted based on a sheep model. In vitro tests were performed based on the chondrocyte cells with the Alcian Blue and safranin O staining in order to prove the presence of proteoglycan on the surface of the synthesized substrate, which has been secreted by cultures of chondrocytes. Furthermore, the expression of collagen type I, collagen type II, aggrecan, and Sox9 was presented in the chondrocyte cultures on the synthesized substrate through RT-PCR. In addition, the H&E analysis and other related tests demonstrated the formation of neocartilage tissue in a sheep model. The results were found to be promising for cartilage tissue engineering and verified that the isolated chondrocyte cultures on the synthesized substrate retain their original composition.
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| http://dx.doi.org/10.1002/jbm.b.35087 | DOI Listing |
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