Impact of Different Diets on Adult Tri-Spine Horseshoe Crab, .

Effective culture and management of adult tri-spine horseshoe crab, can ensure that stock enhancement programs and aquaculture systems are maintained. To explore suitable feed for animals during the breeding season, Pacific oyster () (oyster group; OG) and frozen sharpbelly fish () (frozen fish group; FG) were selected to feed 20 male and female pairs, respectively. At the end of the experiment, intestinal samples were obtained to measure digestive enzymes activities. The intestinal flora were determined by 16S rDNA sequencing. No eggs were observed in the FG and one adult died. No animals died in the OG, and 9.7 × 10 eggs were obtained. These results show that oysters are more suitable for the development and reproduction of adult than frozen fish. Additionally, the digestive enzyme activity analysis revealed that animals in the OG exhibited higher protein digestibility than those in the FG, but no significant differences in lipid and carbohydrate uptake were observed between the groups. Furthermore, the intestinal flora analysis showed that operational taxonomic units (OTUs) and the Chao1 index were significantly higher in the OG than in the FG, but no significant difference was observed in the Shannon or Simpson indices between the groups. Our data indicate that the oyster diet improved the intestinal microbial diversity of . We hypothesize that nutrients, such as oyster-based taurine, proteins, and highly unsaturated fatty acids, improve protease activity in the digestive tract, alter the intestinal floral structure, and improve the reproductive performance of .