AI Article Synopsis
- - DNA methylation plays a crucial role in the development of colorectal cancer (CRC) and analyzing specific methylated genes could provide valuable diagnostic insights.
- - Researchers utilized the Illumina Infinium Human Methylation 850K BeadChip to study genome-wide methylation patterns in early CRC and adenoma patients, revealing significant differences in 1865 methylated CpG sites.
- - The identified methylated sites were distributed across all chromosomes and were linked to important biological functions through enrichment analyses, indicating their potential as biomarkers in CRC detection.
Article Abstract
Background: DNA methylation is a part of epigenetic modification, that is closely related to the growth and development of colorectal cancer (CRC). Specific methylated genes and methylated diagnostic models of tumors have become current research focuses. The methylation status of circulating DNA in plasma might serve as a potential biomarker for CRC.
Aim: To investigate genome-wide methylation pattern in early CRC using the Illumina Infinium Human Methylation 850K BeadChip.
Methods: The 850K Methylation BeadChip was used to analyze the genome-wide methylation status of early CRC patients ( = 5) and colorectal adenoma patients ( = 5). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enrichment analyses were performed on the selected differentially methylated sites to further discover candidate methylation biomarkers in plasma.
Results: A total of 1865 methylated CpG sites with significant differences were detected, including 676 hypermethylated sites and 1189 hypomethylated sites. The distribution of these sites covered from the 1 to 22 chromosomes and are mainly distributed on the gene body and gene promoter region. GO and KEGG enrichment analysis showed that the functions of these genes were related to biological regulation, molecular binding, transcription factor activity and signal transduction pathway.
Conclusion: The study demonstrated that the Illumina Infinium Human Methylation 850K BeadChip can be used to investigate genome-wide methylation status of plasma DNA in early CRC and colorectal adenoma patients.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048525 | PMC |
| http://dx.doi.org/10.4251/wjgo.v14.i4.935 | DOI Listing |
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