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Optimizing Microneutralization and IFN-γ ELISPOT Assays to Evaluate Mpox Immunity.
Vaccines (Basel)
December 2024
Division of Infectious Diseases, Allergy and Immunology, Saint Louis University, St. Louis, MO 63104, USA.
Background: Available assays to measure pox virus neutralizing antibody titers are laborious and take up to 5 days. In addition, assays to measure T cell responses require the use of specific antigens, which may not be the same for all pox viruses. This study reports the development of robust assays for the measurement of mpox-specific neutralizing antibodies and IFN-γ-producing T-cell responses.
View Article and Find Full Text PDFEvaluation of three diagnostic algorithms to reduce normal scan rates, radiation exposure and costs in patients with suspected chronic coronary syndrome referred for 82Rb-Positron Emission Tomography (Rb-PET).
Open Heart
January 2025
Department of Cardiology, University Hospital Basel, Basel, Switzerland.
Background: The majority of functional ischemia tests in patients with suspected chronic coronary syndromes (CCS) yield normal results. Implementing gatekeepers for patient preselection, such as pretest probability (PTP) and/or coronary artery calcium score (CACS), could reduce the number of normal scan results, radiation exposure and costs. However, the efficacy and safety of these approaches remain unclear.
View Article and Find Full Text PDFMonitoring of nonspatial information within working memory in the common marmoset (Callithrix jacchus).
Cereb Cortex
November 2024
Department of Neurology and Neurosurgery, McGill University, Montreal Neurological Institute, 3801 University Street, Montreal, Quebec H3A 2B4, Canada.
The mid-dorsolateral prefrontal cortical region (areas 46 and 9/46) is critical for the monitoring of information in working memory both in the macaque monkey brain and the human brain. The presence of this cytoarchitectonic region in the New World marmoset brain was in debate, but recent anatomical evidence demonstrated a limited area 46. This finding raised the question of the extent to which the marmoset brain can support the cognitive control process of monitoring information within working memory.
View Article and Find Full Text PDFDiagnostic utility of coronary artery calcium score percentiles and categories to exclude abnormal scans and relevant ischemia in rubidium positron emission tomography.
Front Cardiovasc Med
September 2024
Department of Cardiology, University Hospital Basel, University of Basel, Basel, Switzerland.
Background: Despite clinical suspicion, most non-invasive ischemia tests for coronary artery disease (CAD) reveal unremarkable results. Patients with a coronary artery calcium score (CACS) of zero rarely have an abnormal positron emission tomography (PET) and could be deferred from further testing. However, most patients have some extent of coronary calcification.
View Article and Find Full Text PDFNeutrophil-specific expression of JAK2-V617F or CALRmut induces distinct inflammatory profiles in myeloproliferative neoplasia.
J Hematol Oncol
June 2024
Healthcampus Immunology, Inflammation and Infectiology (GC-I, Otto-von-Guericke-University, Magdeburg, Germany.
Background: Neutrophils play a crucial role in inflammation and in the increased thrombotic risk in myeloproliferative neoplasms (MPNs). We have investigated how neutrophil-specific expression of JAK2-V617F or CALRdel re-programs the functions of neutrophils.
Methods: Ly6G-Cre JAK2-V617F and Ly6G-Cre CALRdel mice were generated.
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