AI Article Synopsis
- - The study aimed to determine if human oral mucosal fibroblasts (HOMF) from patients with limbal stem cell deficiency (LSCD) can be utilized to help grow epithelial cells for potential clinical applications.
- - Researchers isolated HOMF from biopsies of LSCD patients with various conditions and compared their effectiveness in supporting human limbal epithelial cells (HLE) against fibroblasts from non-LSCD donors and a standard cell line.
- - Results indicated that HOMF from LSCD patients could support the growth of HLE just as well as control fibroblasts, suggesting that these cells could be valuable in clinical settings for treating LSCD.
Article Abstract
Purpose: To investigate if human oral mucosal fibroblasts (HOMF) from patients with limbal stem cell deficiency (LSCD) can be used as an autologous feeder layer to support the culture of epithelial cells for potential clinical use.
Methods: HOMF were isolated from oral mucosal biopsies obtained from the following groups of patients with LSCD: aniridia, mucous membrane pemphigoid (MMP), Stevens-Johnson syndrome (SJS), and ectodermal dysplasia (ED). The ability of these cells to support the culture of human limbal epithelial cells (HLE) was compared to that of HOMF from non-LSCD donors and 3T3s commonly used to culture epithelial cells for use in the clinic to treat LSCD.
Results: HOMF were successfully obtained by explant culture for 3/3 aniridia patients, 3/3 MMP patients, 1/3 SJS patients, and 1/1 ED patients. All HOMF cultured from these LSCD groups supported the expansion of HLE with epithelial culture times and total colony forming efficiency (CFE) comparable to those achieved on HOMF isolated from donors without LSCD. PCR showed that all HLE cultured on LSCD donor HOMF expressed p63α, CK15, PAX6, CK12, and MUC16 as did HLE cultured on the control non-LSCD donor HOMF and 3T3s. Western blotting detected CK15 and MUC16 protein expression in all groups.
Conclusions: HOMF from patients with LSCD can be successfully used to support the expansion of epithelial cells. These cells may therefore be useful as autologous feeder fibroblasts for the expansion of epithelial cells for use in the clinic to treat LSCD.
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| http://dx.doi.org/10.1080/02713683.2022.2071944 | DOI Listing |
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