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Exploring the use of Congo red agar in improving the serotyping of non-serotypeable Shigella with In-silico evidence.
Indian J Med Microbiol
June 2023
Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Institute and Research (JIPMER), Puducherry, India. Electronic address:
Objectives: To assess if congo red could make non-serotypeable Shigella strains serotypeable and to employ molecular docking to determine the basis of the same phenomenon.
Methods: We used 42 bacterial strains of non-serotypeable Shigella collected from 2012 to 2019 for this study. Each bacterial strain was freshly inoculated onto congo red agar and incubated at 37° C for 18-24 h.
Genomic and Evolutionary Features of Nine AHPND Positive Vibrio parahaemolyticus Strains Isolated from South American Shrimp Farms.
Microbiol Spectr
August 2023
Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.
Vibrio parahaemolyticus is a bacterial pathogen that becomes lethal to shrimps when acquiring the pVA1-type plasmid carrying the PirAB genes, causing acute hepatopancreatic necrosis disease (AHPND). This disease causes significant losses across the world, with outbreaks reported in Southeast Asia, Mexico, and South America. Virulence level and mortality differences have been reported in isolates from different locations, and whether this phenomenon is caused by plasmid-related elements or genomic-related elements from the bacteria remains unclear.
View Article and Find Full Text PDFHighly-conserved regulatory activity of the ANR family in the virulence of diarrheagenic bacteria through interaction with master and global regulators.
Sci Rep
April 2023
Child Health Research Center, Department of Pediatrics, University of Virginia School of Medicine, 409 Lane Road, MR-4 Building, P.O. Box 801326, Charlottesville, VA, 22908, USA.
ANR (AraC negative regulators) are a novel class of small regulatory proteins commonly found in enteric pathogens. Aar (AggR-activated regulator), the best-characterized member of the ANR family, regulates the master transcriptional regulator of virulence AggR and the global regulator HNS in enteroaggregative Escherichia coli (EAEC) by protein-protein interactions. On the other hand, Rnr (RegA-negative regulator) is an ANR homolog identified in attaching and effacing (AE) pathogens, including Citrobacter rodentium and enteropathogenic Escherichia coli (EPEC), sharing only 25% identity with Aar.
View Article and Find Full Text PDFEnzymatic Specificity of Conserved Rho GTPase Deamidases Promotes Invasion of Vibrio parahaemolyticus at the Expense of Infection.
mBio
August 2022
Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
Vibrio parahaemolyticus is among the leading causes of bacterial seafood-borne acute gastroenteritis. Like many intracellular pathogens, V. parahaemolyticus invades host cells during infection by deamidating host small Rho GTPases.
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