AI Article Synopsis
- Research shows that while transcription factors are important for the maintenance and differentiation of pluripotent stem cells (iPSCs/ESCs), posttranscriptional regulatory mechanisms have not been thoroughly examined.
- By profiling both the transcriptome and proteome, the study identified 228 genes that are posttranscriptionally regulated, with significant upregulation of protein levels in iPSCs/ESCs.
- Among these, 20 specific genes were found crucial for iPSC survival, highlighting that posttranscriptional regulation is essential for the viability of these stem cells, especially compared to differentiated cells.
Article Abstract
The effects of transcription factors on the maintenance and differentiation of human-induced or embryonic pluripotent stem cells (iPSCs/ESCs) have been well studied. However, the importance of posttranscriptional regulatory mechanisms, which cause the quantitative dissociation of mRNA and protein expression, has not been explored in detail. Here, by combining transcriptome and proteome profiling, we identified 228 posttranscriptionally regulated genes with strict upregulation of the protein level in iPSCs/ESCs. Among them, we found 84 genes were vital for the survival of iPSCs and HDFs, including 20 genes that were specifically necessary for iPSC survival. These 20 proteins were upregulated only in iPSCs/ESCs and not in differentiated cells derived from the three germ layers. Although there are still unknown mechanisms that downregulate protein levels in HDFs, these results reveal that posttranscriptionally regulated genes have a crucial role in iPSC survival.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097716 | PMC |
| http://dx.doi.org/10.1016/j.isci.2022.104289 | DOI Listing |
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