Introduction: Previous studies have shown an increase of T cells and chemokines in vascular lesions of patients with chronic thromboembolic pulmonary hypertension (CTEPH). However, detailed characterization of these T cells is still lacking, nor have treatment effects been evaluated.
Methods: We included 41 treatment-naive CTEPH patients at diagnosis, 22 patients at 1-year follow-up, and 17 healthy controls (HCs). Peripheral blood T cells were characterized by flow cytometry for subset distribution, cytokine expression and activation marker profile. We used multiplex immunofluorescence to identify CCR6 T cells in endarterectomy tissue from 25 patients.
Results: At diagnosis, proportions of CCR6 CD4 T cells were increased in CTEPH patients compared with HCs. Patients displayed a significantly reduced production capacity of several cytokines including TNFα, IFNγ, GM-CSF and IL-4 in CD4 T cells, and TNFα and IFNγ in CD8 T cells. CD4 and CD8 T cells showed increased expression of the immune checkpoint protein CTLA4. Multivariate analysis separated CTEPH patients from HCs, based on CCR6 and CTLA4 expression. At 1-year follow-up, proportions of CCR6CD4 T cells were further increased, IFNγ and IL-17 production capacity of CD4 T cells was restored. In nearly all vascular lesions we found substantial numbers of CCR6 T cells.
Conclusion: The observed increase of CCR6 T cells and modulation of the IFNγ and IL-17 production capacity of circulating CD4 T cells at diagnosis and 1-year follow-up - together with the presence of CCR6 T cells in vascular lesions - support the involvement of the Th17-associated CCR6 T cell subset in CTEPH.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9094486 | PMC |
http://dx.doi.org/10.3389/fimmu.2022.861450 | DOI Listing |
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