Isolation, Characterization, and Metabolic Profiling of from the Aquatic Plant .

Mycobiology

Botany and Microbiology Department, Faculty of Science, Helwan University, Cairo, Egypt.

Published: April 2022

The goal of the present study was to investigate the antibacterial properties, enzyme production, and metabolic profiling of a new strain isolated from the submerged aquatic plant . Furthermore, the fungus' morphological characterization and DNA sequencing have been described. The fungus has been identified and submitted to the GenBank as isolate EG19 and the fungus ID is MK387081. The enzyme analyses showed its ability to produce protease and cellulase enzymes. According to the CSLI standard, the ethyl acetate extract of showed intermediate antibacterial activity against , , and . Metabolic profiling has been carried out using 700 MHz NMR spectroscopy. Based on the H and H-C heteronuclear single quantum coherence (HSQC) NMR data and NMR databases, 23 compounds have been identified. The identified metabolites include 31% amino acids, 9% sugars, 9% amines, 4% sugar alcohols, and 4% alkaloids. This is the first report for the metabolic characterization of , which gave preliminary information about the fungus. It is expected that our findings not only will pave the way to other perspectives in enormous applications using as a new promising source of antimicrobial agents and essential metabolites, but also it will be valuable in the classification and chemotaxonomy of the species.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068000PMC
http://dx.doi.org/10.1080/12298093.2022.2059889DOI Listing

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