Amyloid fibrils have many excellent functional properties that facilitate their applications in the food industry. There are 2 pathways for whey protein concentrate (WPC) to form amyloid fibril aggregates: spontaneous pathway and nuclear induction pathway. Low ionic strength is a necessary condition for the spontaneous pathway to proceed successfully. In this paper, the effect of salt ions on 2 WPC fibrillation pathways was investigated by adding CaCl. The results demonstrated WPC fibrils were unable to form normally through spontaneous pathway as adding CaCl; but still could form through nuclear induction pathway with 20 to 30 mM CaCl, the nuclei accelerated the fibrillation process led to the resistance to the disordered aggregation brought by CaCl. Moreover, divalent cations (Ca, Mg) had much stronger effects than monovalent cations (Na) on fibril formation, and the results of X-ray photoelectron spectrum together with Fourier-transform infrared spectroscopy suggested that Ca had a greater effect on the fibril formation than Cl.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3168/jds.2021-20895 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
SMARCA4 regulates the NK-mediated killing of senescent cells.
Sci Adv
January 2025
MRC Laboratory of Medical Sciences (LMS), Du Cane Road, London W12 0NN, UK.
Induction of senescence by chemotherapeutic agents arrests cancer cells and activates immune surveillance responses to contribute to therapy outcomes. In this investigation, we searched for ways to enhance the NK-mediated elimination of senescent cells. We used a staggered screen approach, first identifying siRNAs potentiating the secretion of immunomodulatory cytokines to later test for their ability to enhance NK-mediated killing of senescent cells.
View Article and Find Full Text PDFStress Granule Induction in Rat Retinas Damaged by Constant LED Light.
Invest Ophthalmol Vis Sci
January 2025
Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica Ranwel Caputto. Córdoba, Argentina.
Purpose: Stress granules (SGs) are cytoplasmic biocondensates formed in response to various cellular stressors, contributing to cell survival. Although implicated in diverse pathologies, their role in retinal degeneration (RD) remains unclear. We aimed to investigate SG formation in the retina and its induction by excessive LED light in an RD model.
View Article and Find Full Text PDFExercise intensity and training alter the innate immune cell type and chromosomal origins of circulating cell-free DNA in humans.
Proc Natl Acad Sci U S A
January 2025
Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305.
Exercising regularly promotes health, but these benefits are complicated by acute inflammation induced by exercise. A potential source of inflammation is cell-free DNA (cfDNA), yet the cellular origins, molecular causes, and immune system interactions of exercise-induced cfDNA are unclear. To study these, 10 healthy individuals were randomized to a 12-wk exercise program of either high-intensity tactical training (HITT) or traditional moderate-intensity training (TRAD).
View Article and Find Full Text PDFCanonical Wnt signalling from the Area Opaca induces and maintains the Marginal Zone in pre-primitive-streak stage chick embryos.
Development
January 2025
Department of Cell & Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
In chick embryos prior to primitive streak formation, the outermost extraembryonic region, known as the area opaca (AO), was generally thought to act only by providing nutrients and mechanical support to the embryo. Just internal to the AO is a ring of epiblast called the marginal zone (MZ), separating the former from the inner, area pellucida epiblast. The MZ does not contribute cells to any part of the embryo but is involved in determining the position of primitive streak formation from the adjacent area pellucida epiblast.
View Article and Find Full Text PDFInduction of age-related ocular disorders in a mouse model of pulmonary fibrosis.
Exp Eye Res
January 2025
State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University, 270 Xueyuan Road, Wenzhou, Zhejiang 325027, P. R. China. Electronic address:
Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease linked to aging. This study investigates potential connections between IPF and age-related eye problems using a bleomycin-induced IPF mouse model. Intratracheal administration of bleomycin induces rapid lung injury in mice, followed by IPF with characteristics of cellular senescence.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!