Optimization of an Ultra-Sonication Extraction Method for Major Compounds Found in Using Design of Experiment.

Optimum extraction conditions are vital in quality control methods to enable accurate quantification of the compounds of interest. An ultra-sonication method was developed for the extraction of seven major compounds found in . Extraction temperature, time, power, frequency, percentage of ethanol in water and solvent to sample ratio were screened to access their significance on the percentage recovery of the compounds of interest. These parameters were screened using Descriptive screening design. Extraction temperature, solvent to sample ratio and the interaction between temperature and percentage ethanol in water were found to have a significant effect on the response. These parameters were then optimized using central composite design. The optimum conditions were found to be 66.1% ethanol in water, 70 °C temperature and 3 mL: 5 mg solvent to sample ratio. This method was successfully applied in the development of a quality control method for the seven compounds in samples.