Int J Mol Sci
Instituto Universitario Fernández-Vega, Fundación de Investigación Oftalmológica, Universidad de Oviedo, 33012 Oviedo, Spain.
Published: April 2022
The skin is the largest organ in the human body, comprising the main barrier against the environment. When the skin loses its integrity, it is critical to replace it to prevent water loss and the proliferation of opportunistic infections. For more than 40 years, tissue-engineered skin grafts have been based on the in vitro culture of keratinocytes over different scaffolds, requiring between 3 to 4 weeks of tissue culture before being used clinically. In this study, we describe the development of a polymerizable skin hydrogel consisting of keratinocytes and fibroblast entrapped within a fibrin scaffold. We histologically characterized the construct and evaluated its use on an in vivo wound healing model of skin damage. Our results indicate that the proposed methodology can be used to effectively regenerate skin wounds, avoiding the secondary in vitro culture steps and thus, shortening the time needed until transplantation in comparison with other bilayer skin models. This is achievable due to the instant polymerization of the keratinocytes and fibroblast combination that allows a direct application on the wound. We suggest that the polymerizable skin hydrogel is an inexpensive, easy and rapid treatment that could be transferred into clinical practice in order to improve the treatment of skin wounds.
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http://dx.doi.org/10.3390/ijms23094837 | DOI Listing |
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December 2024
College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, 610065, China.
Polymerizable deep eutectic solvents (PDESs) have emerged as promising building blocks for next-generation eutectic gels, offering new opportunities for the development of advanced electronic devices. Traditional PDES fabrication typically involves heating and extended processing time. In this study, a facile method where a solid-solid mixture of lithium bis(trifluoromethane) sulfonimide (LiTFSI) and acrylamide (AAm) rapidly forms a PDES at room temperature, significantly simplifying the ionogel preparation is presented.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
School of Chemistry Materials Engineering, Zhejiang A&F University, Zhejiang Province, Hangzhou 10341, PR China.
Using deep eutectic solvents (DES) to pretreat wheat straw (WS) and extract lignin-containing nanocellulose (LCNC). Acrylic acid/choline chloride (AA/ChCl) polymerizable deep eutectic solvents (PDES) were used as the primary polymerization network, combined with polyvinyl alcohol (PVA). Lignocellulose nanocrystals (LCNC) oxidized by sodium periodate were prepared as dialdehyde-based nanocellulose (DCNC) to serve as the crosslinking agent.
View Article and Find Full Text PDFAdv Healthc Mater
July 2024
College of Marine Life Sciences, Ocean University of China, No. 5 Yushan Road, Qingdao, 266003, China.
Burns can cause severe damage to the skin due to bacterial infection and severe inflammation. Although conductive hydrogels as electroactive burn-wound dressings achieve remarkable effects on accelerating wound healing, issues such as imbalance between their high conductivity and mechanical properties, easy dehydration, and low transparency must be addressed. Herein, a double-network conductive eutectogel is fabricated by integrating polymerizable deep eutectic solvents (PDESs)including acrylamide/choline chloride/glycerol (acrylamide-polymerization crosslink) and thiolated hyaluronic acid (disulfide-bonding crosslink).
View Article and Find Full Text PDFInt J Biol Macromol
April 2024
School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, 255000, China; Shandong Research Center of Engineering and Technology for Clean Energy, Zibo, 255000, China. Electronic address:
Liquid free ion-conductive elastomers (ICEs) have demonstrated promising potential in various advanced application scenarios including sensor, artificial skin, and human-machine interface. However, ICEs that synchronously possess toughness, adhesiveness, stability, and anti-bacterial capability are still difficult to achieve yet highly demanded. Here, a one-pot green and sustainable strategy was proposed to fabricate multifunctional ICEs by extracting non-cellulose components (mainly lignin and hemicellulose) from lignocellulose with polymerizable deep eutectic solvents (PDES) and the subsequent in-situ photo-polymerization process.
View Article and Find Full Text PDFJ Control Release
April 2024
Key Laboratory of Cluster Science of Ministry of Education, Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering of Ministry of Industry and Information Technology, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China. Electronic address:
Microneedle patches are emerging multifunctional platforms for transdermal diagnostics and drug delivery. However, it still remains challenging to develop smart microneedles integrated with customization, sensing, detection and drug delivery by 3D printing strategy. Here, we present an innovative but facile strategy to rationally design and fabricate multifunctional eutectogel microneedle (EMN) patches via multi-material 3D printing.
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