AI Article Synopsis
- The pathogen studied is an opportunistic bacteria that poses severe risks for patients with cystic fibrosis and chronic granulomatous disease.
- Researchers discovered that a specific isolate of this bacteria, H111, can survive without oxygen, which is unusual for an obligate aerobe.
- Through transposon sequencing, 71 factors essential for survival in anoxic environments were identified, with specific genes linked to virulence and movement, underscoring their importance in the bacteria's lifecycle and pathogenicity.
Article Abstract
is an opportunistic pathogen that can lead to severe infections in patients suffering from cystic fibrosis (CF) and chronic granulomatous disease. Being an obligate aerobe, is unable to grow in the absence of oxygen. In this study, we show that the CF isolate H111 can survive in the absence of oxygen. Using a transposon sequencing (Tn-seq) approach, we identified 71 fitness determinants involved in anoxic survival, including a Crp-Fnr family transcriptional regulatory gene (), genes coding for the sensor kinase RoxS and its response regulator RoxR, the sigma factor for flagella biosynthesis (FliA) and subunits of a cytochrome oxidase (CydA, CydB and the potentially novel subunit CydP). Individual knockouts of these fitness determinants significantly reduced anoxic survival, and inactivation of both copies is shown to be lethal under anoxic conditions. We also show that the two-component system RoxS/RoxR and FliA are important for virulence and swarming/swimming, respectively.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104464 | PMC |
| http://dx.doi.org/10.3390/ijms23094560 | DOI Listing |
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