AI Article Synopsis
Article Abstract
Objective: To evaluate the effect of storage of ovaries before cryopreservation on long-term fertility and ovarian reserve after transplantation in mice.
Design: Experimental study.
Setting: University hospital.
Animal(s): C57BL/6J and C57BL/6J-Tg (CAG-GFP) female mice.
Intervention(s): Storage and cryopreservation of mouse ovaries. Long-term fertility analysis of mice transplanted with thawed ovaries.
Main Outcome Measure(s): Estrous cycles, number of live births, ovarian weight, and follicular counts of ovarian grafts.
Result(s): At the first mating 3 months after ovarian transplantation, the mean number of live births was 2.6 ± 0.6 in the control group (no storage); in the storage groups, the mean number of live births was 2.9 ± 0.7 after 4 hours, 1.3 ± 0.5 after 8 hours, 0.2 ± 0.2 after 12 hours, and 0.8 ± 0.5 after 24 hours of storage; the difference from the control group was significant in the 12-hour storage group. At the second mating 6 months after ovarian transplantation, the mean number of live births was 1.8 ± 0.6 in the control group and 2.4 ± 0.6 and 0.3 ± 0.2 in the 4- and 8-hour storage groups, respectively; no live births occurred in the 12- and 24-hour storage groups. Seven months after ovarian transplantation, the numbers of primordial, primary, early secondary, and late secondary follicles were significantly lower in the 8-, 12-, and 24-hour storage groups than in the control group.
Conclusion(s): In mice, shortening the storage time of ovaries before cryopreservation preserved fertility and ovarian reserve after transplantation, indicating that human ovaries might be cryopreserved immediately after harvesting or transported as quickly as possible to a cryopreservation facility to allow young patients with cancer to preserve long-term fertility and ovarian reserve.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.xfss.2022.02.006 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of phloroglucinol on uterine peristalsis after frozen embryo transfer in patients experiencing recurrent implantation failure: A retrospective case-control study.
Eur J Obstet Gynecol Reprod Biol
December 2024
Reproductive Medicine Center, Affiliated Maternity and Child Health Care Hospital of Nantong University, Jiangsu, China. Electronic address:
Objective: To retrospectively investigate the efficacy of phloroglucinol administration after freeze-thawed embryo transfer in patients experiencing recurrent implantation failure (RIF).
Materials And Methods: A total of 139 infertile couples who experienced RIF undergoing in vitro fertilisation and embryo transfer were divided into the control (n = 66) and the phloroglucinol (n = 73) groups. Women in the phloroglucinol group received 80 mg phloroglucinol 30 min after transfer, then once daily up to day 3 after the embryo transfer.
Effects of pretreatment strategies on fertility outcomes in patients with adenomyosis.
Front Reprod Health
December 2024
Department of Obstetrics and Gynaecology, Fribourg University Hospital, Fribourg, Switzerland.
Adenomyosis is a commonly encountered pathology in women of reproductive age and frequently coexists with infertility. The effect of adenomyosis on fertility, particularly on fertilisation and intracytoplasmic sperm injection outcomes, is not well understood. Various pretreatment modalities have been used to improve pregnancy rates and live birth outcomes; however, because of a lack of high-quality evidence, there is no clear consensus on the best pretreatment option.
View Article and Find Full Text PDFHow Can Outdoor Air Pollutants Adversely Affect the Women's Fertility? Systematic Review.
Adv Biomed Res
November 2024
Vice Chancellery for Research and Technology, Isfahan University of Medical Sciences, Isfahan, Iran.
In the current century, air pollution is known as one of the most critical environmental problems and it is important to find the relations of air pollution and human health. Various air pollutants, such as volatile organic compounds (VOCs), can negatively affect women's fertility. An exhaustive electronic search was done from 2013 until July 2023 in PUBMED and The Cochrane Central Register of Controlled Trials.
View Article and Find Full Text PDFState-wise variation and inequalities in caesarean delivery rates in India: analysis of the National Family Health Survey-5 (2019-2021) data.
Lancet Reg Health Southeast Asia
January 2025
The George Institute for Global Health, New Delhi, India.
Background: India's caesarean delivery (CD) rate of 21.5% suggests adequate national access to CD but may mask significant disparities. We examined variation in CD rates across states (geography), wealth, and health care sector (public versus private).
View Article and Find Full Text PDF[Increasing the cumulative live birth rate : low-grade blastocysts, potential overlook].
Gynecol Obstet Fertil Senol
December 2024
Service de Médecine et Biologie de la reproduction, Centre Hospitalier Universitaire, Hôpital Arnaud de Villeneuve, 371, avenue du Doyen-Gaston-Giraud, 34090 Montpellier cedex 5, France. Electronic address:
It is now widely recognized that, following prolonged culture, the transfer of a high-quality morphologically graded blastocyst is the preferred strategy in embryo transfer. Low-grade blastocysts are often considered to have a low implantation potential, and their use remains highly limited. We conducted a general review of the literature, including publications from August 2017 to October 2023, to assess the current state of knowledge regarding these embryos, which are generally excluded in routine practice.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!