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Purpose: To dissect the tumor ecosystem following immune checkpoint blockades (ICBs) in intrahepatic cholangiocarcinoma (ICC) at a single-cell level.
Methods: Single-cell RNA sequencing (scRNA-seq) data of 10 ICC patients for the ICB clinical trial were extracted from GSE125449 and systematically reanalyzed. Bulk RNA-seq data of 255 ICC patients were analyzed. Infiltration levels of SPP1CD68 tumor-associated macrophages (TAMs) were examined by dual immunofluorescence (IF) staining in 264 resected ICC samples. The correlation between SPP1 TAMs and clinicopathological features as well as their prognostic significance was evaluated.
Results: Among the 10 patients, five received biopsy at baseline, and others were biopsied at different timings following ICBs. Single-cell transcriptomes for 5,931 cells were obtained. A tighter cellular communication network was observed in ICB-treated ICC. We found a newly emerging VEGF signaling mediated by PGF-VEGFR1 between cancer-associated fibroblasts (CAFs) and endothelial cells in ICC following ICBs. SPP1 expression was dramatically upregulated, and SPP1 TAM gene signatures were enriched in TAMs receiving ICB therapy. We also identified SPP1 TAMs as an independent adverse prognostic indicator for survival in ICC.
Conclusion: Our analyses provide an overview of the altered tumor ecosystem in ICC treated with ICBs and highlight the potential role of targeting CAFs and SPP1TAMs in developing a more rational checkpoint blockade-based therapy for ICC.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9088915 | PMC |
| http://dx.doi.org/10.3389/fimmu.2022.871769 | DOI Listing |
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