sp. nov., isolated from a tidal flat sediment of the East China Sea.

A Gram-stain-negative bacterial strain, designated as E165, was isolated from a tidal flat sediment of the East China Sea. Strain E165 grew optimally at pH 6, at 32 °C and with 1-2 % (w/v) NaCl. The 16S rRNA gene sequence similarity results revealed that strain E165 was most closely related to IVIA-Po-145, CK13, IVIA-Po-155, HDW-15, 46-16, B20-1 and DSL-35 with values of 97.0-98.5 %. The digital DNA-DNA hybridization and average nucleotide identity values between strain E165 and the reference strains were 21.9-34.3 % and 77.6-87.3 %, respectively. The DNA G+C content of the isolate was 42.9 mol%. Strain E165 contained Q-8 as the sole ubiquinone and C, summed feature 8 (C 7 and/or C 6) and summed feature 3 (C 7 and/or C 6) as the major fatty acids. The major polar lipids of strain E165 were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, aminolipid and aminophospholipid. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness, a novel species, sp. nov., is proposed with E165 (=MCCC 1K06241=KCTC 82809) as the type strain.