AI Article Synopsis
- Researchers used CRISPR technology to create engineered microbes that can store and record information from their environment, particularly gene expression changes.
- These microbes can capture significant interactions between the host and its gut environment, including responses to dietary changes, inflammation, and the complexity of the gut microbiome.
- The study employed Record-seq to track the transcriptional history of bacterial strains in the intestines, allowing for a deeper understanding of how genetic variations impact microbial adaptation without altering the host's physiology.
Article Abstract
Transcriptional recording by CRISPR spacer acquisition from RNA endows engineered with synthetic memory, which through Record-seq reveals transcriptome-scale records. Microbial sentinels that traverse the gastrointestinal tract capture a wide range of genes and pathways that describe interactions with the host, including quantitative shifts in the molecular environment that result from alterations in the host diet, induced inflammation, and microbiome complexity. We demonstrate multiplexed recording using barcoded CRISPR arrays, enabling the reconstruction of transcriptional histories of isogenic bacterial strains in vivo. Record-seq therefore provides a scalable, noninvasive platform for interrogating intestinal and microbial physiology throughout the length of the intestine without manipulations to host physiology and can determine how single microbial genetic differences alter the way in which the microbe adapts to the host intestinal environment.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11163514 | PMC |
| http://dx.doi.org/10.1126/science.abm6038 | DOI Listing |
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