Microbial keratinases exhibit a momentous role in converting keratin biowastes into exceedingly valuable protein supplements. This study reports a novel, highly stable keratinase from RSA27 for the production of pure peptides rich in essential amino acids from chicken feathers. Purified keratinase showed a specific activity of 38.73 U/mg, 2.58-fold purification, and molecular weight of 36 kDa. Kinetic studies using a chicken feather as substrate report and values of 5.69 mg/ml and 142.40 μg/ml/min, respectively, suggesting significant enzyme-substrate affinity/biocatalysis. Identification and structural-functional analysis of keratinase discovered the presence of distinct amino acid residues and their positions. Besides, keratinase possesses a high-affinity calcium-binding site (Asp, Leu, Asn, Ile, and Val) and a catalytic triad of Asp, His, and Ser, known attributes of serine protease (subtilisin family). Furthermore, a scale-up to 5 L fermenter revealed complete feather hydrolysis (94.5%) within 24 h with high activity (789 U/ml) and total amino acid of 153.97 μmol/ml. Finally, cytotoxicity evaluation of protein hydrolysate resulted in negligible cytotoxic effects (1.02%) on the mammalian hepatoblastoma cell line, signifying its potential biotechnological applications.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9083118 | PMC |
| http://dx.doi.org/10.3389/fmicb.2022.882902 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of naked neck and frizzle genes on growth and egg-laying performance of chickens in the tropics in an era of climate change.
Int J Biometeorol
January 2025
Department of Biochemistry and Biotechnology, Faculty of Biosciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
In regions characterized by tropical and subtropical climates, the elevated ambient temperatures exert adverse effects on both broiler and laying chickens, impacting their growth and egg production performance. To mitigate the challenges posed by heat stress, genetic strategies aimed at reducing feather coverage have gained prominence in hot climate areas. Among these approaches, the naked neck (Na) and frizzle (F) genes have emerged as particularly noteworthy.
View Article and Find Full Text PDFIsolation, characterization, and mycostimulation of fungi for the degradation of polycyclic aromatic hydrocarbons at a superfund site.
Biodegradation
January 2025
Civil and Environmental Engineering, Duke University, Hudson Hall 121, Box 90287, Durham, NC, 27708, USA.
Mycoremediation is a biological treatment approach that relies on fungi to transform environmental pollutants into intermediates with lower environmental burden. Basidiomycetes have commonly been used as the target fungal phylum for bioaugmentation in mycoremediation, however this phylum has been found to be unreliable when used at scale in the field. In this study, we isolated, characterized, and identified potential polycyclic aromatic hydrocarbon (PAH) degrading fungal isolates from creosote-contaminated sediment in the Elizabeth River, Virginia.
View Article and Find Full Text PDFImpact of fermented bamboo powder on the morphology and physiology of the gastrointestinal tract in yellow-feather broiler chickens.
Poult Sci
January 2025
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:
Bamboo powder, a novel ingredient, is gaining recognition for its potential as a dietary supplement in poultry feed. This study aimed to investigate the effects of fermented bamboo powder (FBP) on antioxidant status, gut hormone activities, intestinal digestive enzyme activities, gut morphological structure, gastrointestinal development, and the expression of nutritional transporter genes in dwarf yellow-feather broiler chickens. A total of 600 healthy 1-day-old chicks were allocated randomly into two groups, with 10 replicates per group and 30 chicks in each replicate.
View Article and Find Full Text PDFTransforming Feather Meal Into a High-Performance Feed for Broilers.
Vet Med Sci
January 2025
Department of Industrial Management, Faculty of Humanities, University of Tehran, Kish International Campus, Tehran, Iran.
Background: The poultry industry faces challenges with the high cost and environmental impact of Soybean meal. Feather meal, a byproduct with low digestibility due to its keratin content, is a potential alternative. Recent biotechnological advances, including enzymatic and bacterial hydrolysis, have enhanced its digestibility and nutritional value.
View Article and Find Full Text PDFFeeding citrus pomace fermented with combined probiotics improves growth performance, meat quality, fatty acid profile, and antioxidant capacity in yellow-feathered broilers.
Front Vet Sci
December 2024
State Key Laboratory of Swine and Poultry Breeding Industry, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangdong Engineering Technology Research Center of Animal Meat Quality and Safety Control and Evaluation, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Introduction: The reasonable and efficient utilization of agricultural by-products as animal feed has the capacity to not only mitigate the scarcity of conventional feedstuff but also alleviate the environmental load. This study was aimed to investigate the effects of feeding citrus pomace (CP) fermented with combined probiotics on growth performance, carcass traits, meat quality and antioxidant capacity in yellow-feathered broilers.
Methods: A cohort of 540 female yellow-feathered broilers (Qingyuan partridge chicken, 90-day-old) were randomly divided into three groups and, respectively, fed the basal diet (Control), diet containing 10% unfermented CP (UFCP) and diet containing 10% fermented CP (FCP).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!