The aim of this study was to investigate the efficacy of a skin substitute composed of mPEG-PCL-grafted-gelatin (Bio-Syn)/hyaluronan/chondroitin sulfate/sericin and to study its biocompatibility with human fibroblasts, human keratinocytes and hMSCs in terms of cellular adhesion and proliferation (∼5-6 fold). mPEG-PCL was grafted into a gelatin backbone a Michael addition reaction to prepare Bio-Syn and it was characterized using ATR-FTIR, H NMR and TNBS assay. Additionally, keratinocyte-hMSC contact co-culture studies showed that Bio-Syn composite scaffolds loaded with sericin promote hMSCs' epithelial differentiation with regard to qRT-PCR gene expression (ΔNp63α and keratin 14) and expression of various epithelial markers (Pan-cytokeratin, ΔNp63α and keratin 14). efficacy studies on a 2 degree burn wound model in Wistar rats showed an improved rate of wound contraction, histology (H&E and Van Gieson's staining) and pro-healing marker (hexosamine, hydroxyproline, ) expression in granular tissue compared to using the commercial dressing Neuskin™ and a cotton gauze control.
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| http://dx.doi.org/10.1039/c8ra01489b | DOI Listing |
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