Protocols for measuring phosphorylation, subcellular localization, and kinase activity of Hippo pathway components YAP and LATS in cultured cells.

The Hippo pathway plays critical roles in cell growth, differentiation, development, tissue homeostasis, and cancer. Here, we describe protocols to measure phosphorylation, subcellular localization, and kinase activity for core Hippo pathway components YAP and LATS. The phos-tag technology is particularly useful to quantitatively detect protein phosphorylation even without a phosphospecific antibody. We use HEK293A cells as an example, but the protocols can be applied to other cell lines and tissues. For complete details on the use and execution of this profile, please refer to Luo et al. (2020).