AI Article Synopsis
- Capturing the dynamics of protein phosphorylation is complex due to its low levels and transient nature.
- This study introduces a sensitive method for measuring virus-induced phosphorylation using label-free quantification-based phosphoproteomics.
- The protocol includes steps like protein digestion, phosphopeptide enrichment, mass spectrometry, and bioinformatics analysis, as detailed in Hunziker et al. (2022).
Article Abstract
Due to the low stoichiometry and highly transient nature of protein phosphorylation it is challenging to capture the dynamics and complexity of phosphorylation events on a systems level. Here, we present an optimized protocol to measure virus-induced phosphorylation events with high sensitivity using label free quantification-based phosphoproteomics. Specifically, we describe filter assisted protein digestion (FASP), enrichment of phosphopeptides, mass spectrometry, and subsequent bioinformatic analysis. For complete details on the use and execution of this protocol, please refer to Hunziker et al. (2022).
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076958 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2021.101089 | DOI Listing |
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